Observation Variable Method List

Name Description Instrument Software Method Class Action
Lenght measurement Measurement method
Two-dimensional digital phenotyping of plant organ morphology and color Colour is measured in the cross section of the fruit using Tomato Analyzer Color Test Tomato Analyzer Computational method
triestimulus colorimetry colorimeter Measurement method
Tomato Analyzer two-dimensional digital phenotyping of transversal section fruit morphology and color Fruits were cut transversally, scanned and stored as digital image. The scanned fruit were analyzed fruit morphology and color Tomato Analyzer Computational method
NaOH titration Measurement method
food components enzymatic test citric acid, malic acid, and glucose/fructose analysis kits (R-Biopharm, Marshall, MI, USA) Measurement method
Visual phenotyping of plant and plant organ characteristics Visual method
Tomato Analyzer two-dimensional digital phenotyping of longitudinal section fruit morphology and color Fruits were cut longitudinally, scanned and stored as digital image. The scanned fruit were analyzed fruit morphology and color Tomato Analyzer Computational method
Weigth measurement Measurement method
Counting of plant and plant organ characteristics Counting method
Percent calculation Calculation
Phenotyping of plant and plant organ characteristics by stereomicroscopy 2 The pericarp slices were stained in 0.15% toluidine blue solution. Variables were measured from the microscopy images using the software ImageJ bundled with 64-bit Java 1.8.0_112 and the MorphoLibJ plugin OLYMPUS MVX10 optical microscope Image J software, MorphoLibJ plugin Measurement method
pH measurement Measurement method
Phenotyping of plant and plant organ characteristics by stereomicroscopy 1 germinated pollen was counted in a stereomicroscope with epi-illumination. The images from the microscope were analyzed with Image J software1 to calculate the pollen number (AB) (number of grains in 200 μM) stereomicroscope with epi-illumination Image J software Counting method
Phenotyping of plant and plant organ characteristics by fluorescence microscopy 1 The aniline blue stain was used to identify and evaluate pollen grains by visualization with a Nikon Eclipse E600 microscope. The images from the microscope were analyzed with Image J software1 to calculate the pollen number (AB) (number of grains in 200 μM) Nikon Eclipse E600 fluorescence microscope Image J software Counting method
brix measurement Measurement method
Phenotyping of plant and plant organ characteristics by flow citometry 1 AmphaTMZ32 flow cytometer Counting method
Firmness measurement durometer Measurement method
difference calculation between two variables Calculation
LC/APCI/MS analysis of non-polar fraction Measurement method
GC-MS TOF analysis of primary metabolites Measurement method
HS-SPME coupled to GC-MS anlaysis of volatiles Measurement method
LC-ESI_PDA_MS analysis of semi-polar fraction Measurement method
Non-structural carbohydrates enzymatic assay method in 96-well microplates (MP method) Enzymatic microplate method to successively quantify glucose, fructose and sucrose from the same extract aliquot, in the same well. Measurement method
log10 transformation the logarithmic transformation of a measured value into log10 scale Calculation
Small-Scale Analysis of Vitamin C Levels analysis of both total and reduced forms of vitamin C ( ascorbate and dehydroascorbate) in fruits using microplates and a plate reader.The technique involves the reduction of iron 3+ to iron 2+ by ascorbate and on the reaction of oxidized ascorbate with orthophenylenediamine Measurement method