Phenotyping of plant and plant organ characteristics by stereomicroscopy 2
The pericarp slices were stained in 0.15% toluidine blue solution. Variables were measured from the microscopy images using the software ImageJ bundled with 64-bit Java 1.8.0_112 and the MorphoLibJ plugin
Phenotyping of plant and plant organ characteristics by stereomicroscopy 1
germinated pollen was counted in a stereomicroscope with epi-illumination. The images from the microscope were analyzed with Image J software1 to calculate the pollen number (AB) (number of grains in 200 μM)
Phenotyping of plant and plant organ characteristics by fluorescence microscopy 1
The aniline blue stain was used to identify and evaluate pollen grains by visualization with a Nikon Eclipse E600 microscope. The images from the microscope were analyzed with Image J software1 to calculate the pollen number (AB) (number of grains in 200 μM)