Observation Variable Method Details

Observation Variable Method Basic Information

Phenotyping of plant and plant organ characteristics by stereomicroscopy 2
The pericarp slices were stained in 0.15% toluidine blue solution. Variables were measured from the microscopy images using the software ImageJ bundled with 64-bit Java 1.8.0_112 and the MorphoLibJ plugin

Instrument, Software & Method Class

OLYMPUS MVX10 optical microscope
Image J software, MorphoLibJ plugin
Measurement method

1) doi: 10.1007/s00122-021-03902-2

Related Observation Variables Summary 2

Name Description Trait
pericarp cell layers, counting Number of cell layers in the tomato pericarp in axial abaxial axis. Counting the number of cells intersected by a line perpendicular to the exocarp, avoiding vascular bundles, endocarp layer, 2-4 small cell layers right bellow the exocarp, and 0-1 layers right above the endocarp. SP:0000397
pericarp max cell size, calculation in square micrometers, um^2 Size of the biggest tomato cells in the pericarp in um^2. ID:0000376