Growth and genotyping by sequencing of the TRADITOM collection representing variability of tradtional tomato cultivated across Shouthern Europe during 1950-2015. Seeds of traditional European tomato accessions which composed the TRADITOM collection, were obtained from the GeneBanks of the Institute for the Conservation and Improvement of Valencian Agrodiversity of the Polytechnic University of Valencia (COMAV-UPV, Valencia, Spain), from the Balearic Island University (UIB, Mallorca, Spain), from the Station d`Amelioration des Plantes Maraicheres of the French National Institute for Agricultural Research, (INRA, Montfavet, France), from the Agrobiology Department Agricultural Faculty of the University of Tuscia (UNITUS, Viterbo, Italy), from the Institute of Biosciences and Bioresources of Italian National Council of Research (CNR-IBBR, Portici, Italy), from the Greek GeneBank of Agricultural Research Center of Macedonia and Thrace of the National Agricultural Research Foundation (GGB-NAGREF, Thessaloniki, Greece) and from the seed collections of the Miquel Agustí Foundation of the Polytechnic University of Catalunya (FMA-UPC, Casteldefels, Spain), from the ARCA Società Cooperativa A.r.l. (ARCA2010, Acerra, Italy), from the University of Reggio Calabria (UNIRC, Regio de Calabria, Italy), and from the Robert H. Smith Faculty of Agriculture, Food and Environment of the Hebrew University of Jerusalem (HUJI-ARO, Rehovot, Israel).
Instituto de Biología Molecular y Celular de Plantas Primo Yufera greenhouses
ESP
Private
greenhouse study
Glass greenhouse
All the seeds were sanitized with a double chemical treatment followed by a thermal treatment as follows: seeds were washed in 10% trisodium phosphate for 3 hours, then washed in 30% bleach for 1 hour, and finally subjected to a dry heat treatment at 79 °C for 24 hours. Seeds were germinated in Petri dishes and transplanted to soil using trays with 60-cell plugs when the cotyledons were fully expanded. Plants were grown in a glass greenhouse until 3–4 true leaves had developed.
Experimental Design Information
Completely randomized design
Five to ten plants per accession were used as sample
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